What is a Prestained Protein Marker IV (8-200 KDa)?

The Prestained Protein Marker IV (8-200 KDa) is a molecular weight ladder used in protein analysis to estimate the size of proteins on SDS-PAGE gel. This marker is pre-stained, allowing researchers to visualize protein migration in real-time during electrophoresis. It contains a range of known protein sizes, making it a reliable reference for determining the molecular weights of your samples. Whether you're running western blots or preparing for mass spectrometry, this marker helps ensure accuracy in identifying proteins.

How do I use the Prestained Protein Marker IV in my experiments?

To use the Prestained Protein Marker IV, simply mix the marker with your sample buffer and load it onto your gel alongside your protein samples. It provides an immediate visual indication of the progress of protein separation as it runs through the gel. This is especially useful for troubleshooting, as you can easily assess the quality of your gels and optimally adjust conditions for subsequent analysis. Practically, it's ideal for researchers looking to confirm the protein sizes in gel electrophoresis.

What are the benefits of using a prestained marker over a standard one?

Prestained markers, like the Prestained Protein Marker IV, provide immediate visualization of protein migration without needing further staining steps post-electrophoresis. This can save time and improve the experimental workflow. Additionally, visualizing the marker helps detect issues during the run, such as improper sample loading or gel integrity. This advancement allows for quicker troubleshooting and improves your overall efficiency in protein analysis, making it a preferred choice for many laboratories.

Can I use the Prestained Protein Marker IV for Western blot analysis?

Yes, the Prestained Protein Marker IV can be used for Western blotting analysis. After SDS-PAGE, the proteins, including the prestained marker, are transferred to a membrane. The visual markers help confirm that proteins have transferred successfully before proceeding with antibody detection. This is especially beneficial for quickly assessing transfer, ensuring that the subsequent steps can be performed with confidence. Researchers often rely on it for its dual role in gel electrophoresis and Western blot applications.

Is the Prestained Protein Marker IV suitable for all types of gels?

The Prestained Protein Marker IV is compatible with most standard polyacrylamide gels used in SDS-PAGE. However, its performance may vary slightly with the gel concentration. Lower percentage gels are better suited for separating low molecular weight proteins, whereas higher percentage gels work well for larger proteins. By selecting the appropriate gel concentration, you enhance the marker's effectiveness, making it an essential tool for accurate molecular weight estimation in protein research.

What concentration should I use for the Prestained Protein Marker IV?

For optimal results, it is generally recommended to load about 5 µL of the Prestained Protein Marker IV alongside your samples on the gel. The concentration may need to vary based on the specific protocol you're following or the quantity of total protein in your samples. Loading the right volume allows for clear visualization and an accurate reference point during analysis. This ensures reliable outcomes, particularly valuable in research settings where precision is key.

Where can I find the specifications of the Prestained Protein Marker IV?

Specifications for the Prestained Protein Marker IV can typically be found on the product's packaging or the manufacturer's website. These specifications include detailed information about the size range (8-200 KDa), unit concentration, and recommendations for use. Additionally, many research-oriented platforms provide user manuals that detail application notes, helping researchers maximize the effectiveness of the marker in their projects.

How does the price of the Prestained Protein Marker IV compare to other similar products?

The Prestained Protein Marker IV is often competitively priced compared to similar markers on the market. Factors affecting the pricing include the range of molecular weights covered and the specific brand reputation. When selecting a protein marker, consider factors such as accuracy, reliability, and compatibility with your experimental protocols. It's important to choose a marker that balances performance with value, ensuring you get the best results for your research needs.

What storage conditions are recommended for the Prestained Protein Marker IV?

The Prestained Protein Marker IV should be stored at -20°C for long-term use. For short-term storage, it can remain stable in a refrigerator at 4°C. Make sure to avoid multiple freeze-thaw cycles, as this could affect the integrity of the proteins within the marker. Proper storage conditions ensure that you maintain its efficacy for accurate protein size estimation in your analyses, making it a reliable choice for lab protocols.

Where can I buy Prestained Protein Marker IV (8-200 KDa) in Tanzania?

You can buy the Prestained Protein Marker IV (8-200 KDa) on Ubuy. This platform offers a wide selection of laboratory products, including various protein markers, ensuring that you can find what you need quickly and conveniently. Ubuy often features a user-friendly interface and reliable delivery options, catering to your research needs in and around Tanzania.

How to Shop Prestained Protein Marker IV (8-200 kDa) Online From Ubuy?

It’s easy to shop Prestained Protein Marker IV (8-200 kDa) online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.

Is Prestained Protein Marker IV (8-200 kDa) Available to Shop Online in Tanzania?

Yes, at Ubuy Tanzania this product is available for you to shop at a reasonable price. The Prestained Protein Marker IV (8-200 kDa) is not available locally but you can trust us with our express shipping services.

How Long Does It Take to Get Product After Placing the Order?

The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

Hygia Reagents

Prestained Protein Marker IV (8-200 kDa)

Convenient for monitoring of electrophoresis and transfer efficiency.

Hygia Reagents

Item #: 105584685

Prestained Protein Marker IV (8-200 kDa)

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Item #: 105584685

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What Stands Out

Wide Range

Covers a broad molecular weight spectrum from 8 to 200 kDa, making it suitable for various protein studies and experiments, ensuring versatility in research applications.

Pre-stained Convenience

Ready-to-use format saves time, allowing researchers to visualize protein bands instantly during gel electrophoresis, enhancing workflow efficiency and eliminating additional staining steps.

High Precision

Delivers accurate, reliable benchmarks for protein migration, ensuring consistent results in Western blotting and other applications, fostering confidence in experimental outcomes and data interpretation.

Product Details

Item Weight

0.5 lbs (230 grams)

Who Should Buy?

Research Laboratories
Ideal for researchers needing accurate protein sizing and visualization in SDS-PAGE experiments with varying protein weights.
Quality Control
Suitable for quality control processes in labs to confirm molecular weight during protein purification and analysis.
Education Purposes
Perfect for educational institutions for teaching protein analysis techniques in a hands-on, practical learning environment.

High Precision Studies
Not ideal for experiments requiring extremely precise molecular weight measurements or specialized protein interactions.

Product Description

Prestained Protein Marker IV (8-200 kDa)

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Question: How to Shop Prestained Protein Marker IV (8-200 kDa) Online From Ubuy?

Answer: It’s easy to shop Prestained Protein Marker IV (8-200 kDa) online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is Prestained Protein Marker IV (8-200 kDa) Available to Shop Online in Tanzania?

Answer: Yes, at Ubuy Tanzania this product is available for you to shop at a reasonable price. The Prestained Protein Marker IV (8-200 kDa) is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Features & Benefits

Contains a mix of eleven high-purity pre-stained recombinant proteins.
Ideal for SDS-PAGE and Western Blot protein molecular weight standard.
Bands range from 8-200 kDa for versatile applications.
Features distinct color coding for easy identification: rose-red at 8, 30, and 90 kDa, blue at others.
Storage at -20°C and valid for 12 months ensures longevity.
Shipped with ice for optimal handling conditions.

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Prestained Protein Marker IV (8-200 kDa)

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Hygia Reagents Natural Proteins Editorial Review

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Important information

TZS 175951

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