PBS [1X] (Phosphate Buffered Saline), 1 Liter
TZS 218891
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1X PBS is a ready-to-use, sterile, pH adjusted solution perfect for your laboratory needs.
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Product Details
| Item Weight | 2.2 lbs (1 kg) |
Who Should Buy?
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Laboratory Researchers
Essential for cell culture, sample dilution, and various assays requiring a stable pH environment in biological studies.
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Medical Professionals
Used in various diagnostic tests and medical procedures requiring a sterile and buffered saline solution.
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Biotechnology Companies
Ideal for production processes involving proteins and antibodies that require consistent buffering in laboratory setups.
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Home Use
Not suitable for household applications due to its specific usage in controlled laboratory environments.
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Animal Consumption
Not intended for ingestion or use in food products; can be harmful if consumed by animals or humans.
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Non-Biological Applications
Lacks efficacy in applications outside of biological sciences like agriculture or industrial use scenarios.
Product Description
PBS [1X] (Phosphate Buffered Saline), 1 Liter
Customer Questions & Answers
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Question:
What is PBS [1X] (Phosphate Buffered Saline) used for?
Answer: PBS [1X] is a sterile solution that maintains a constant pH and osmotic balance for biological experiments. It is widely used in various applications, including cell culture, immunological assays, and molecular biology techniques. The buffered saline helps to stabilize cellular environments, making it crucial for processes like rinsing cells, diluting reagents, or preparing samples for analysis. -
Question:
How is PBS [1X] prepared?
Answer: PBS [1X] can be prepared by diluting a concentrated form of phosphate buffer in distilled water. This involves mixing specific amounts of sodium chloride, potassium chloride, disodium hydrogen phosphate, and potassium dihydrogen phosphate. It’s important to adjust the pH to 7.4 for optimal performance in biological applications. Ready-to-use formulations are widely available for convenience. -
Question:
Is PBS [1X] safe to use in laboratory settings?
Answer: Yes, PBS [1X] is considered safe for use in laboratory environments. It is non-toxic and compatible with a variety of biological systems. However, standard laboratory safety protocols should still be followed. Always handle chemical solutions with appropriate personal protective equipment and proper disposal methods for used materials to ensure a safe working environment. -
Question:
Can PBS [1X] be stored, and if so, how?
Answer: PBS [1X] can be stored at room temperature or refrigerated to prevent microbial growth. It is advisable to keep it in a sterile container to ensure its integrity. If prepared in larger batches, consider aliquoting it into smaller portions for convenient use without compromising the entire solution. Once opened, usage within a reasonable timeframe is recommended to maintain its effectiveness. -
Question:
What is the pH of PBS [1X]?
Answer: The pH of PBS [1X] is typically adjusted to around 7.4, which is optimal for most biological applications. This pH level closely mimics physiological conditions, making it an ideal choice for experiments involving cells or tissues. Maintaining the correct pH is crucial as it significantly affects enzyme activity and cellular processes. -
Question:
Can PBS [1X] be used for washing cells?
Answer: Yes, PBS [1X] is frequently used to wash cells in various laboratory protocols. It helps to remove any residual media or serum components before further processing, such as staining or analysis. Rinsing with PBS ensures that experimental conditions are standardized and that results are reliable, particularly in assays where cell viability or functionality is being assessed. -
Question:
Is PBS [1X] suitable for freezing cells?
Answer: PBS [1X] can be used as a diluent for cryoprotectants, but it is not suitable as a freezing medium by itself. When freezing cells, combine PBS with a cryoprotectant like dimethyl sulfoxide (DMSO) or glycerol that helps preserve cell viability during the freezing process. This combination minimizes ice crystal formation and enhances cell survival upon thawing. -
Question:
What is the difference between PBS [1X] and PBS [10X]?
Answer: PBS [1X] is a diluted version of PBS [10X], which is a concentrated formulation. The main difference lies in their uses: PBS [10X] needs to be diluted to 1X before use in experiments. The concentration ensures you have a longer shelf life and can be prepared freshly as needed. Make sure to adjust the pH after dilution, if necessary. -
Question:
Where can I buy PBS [1X] (Phosphate Buffered Saline), 1 Liter in Tanzania?
Answer: You can purchase PBS [1X] (Phosphate Buffered Saline), 1 Liter from Ubuy, which offers a variety of laboratory supplies and reagents tailored for professionals. Ubuy often features numerous brands and formulations, providing you with options to meet your specific research needs. Check out their inventory to find the best fit for your laboratory.
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Pros
- Great for cell culture
- Stable pH levels
- Easy to use
- Compatible with various applications
- High-quality ingredients
Cons
- Bottle design could be improved.
Product Price History
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TZS 218891
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Features & Benefits
- Ready-to-use sterile solution.
- pH adjusted to 7.4 for optimal performance.
- Contains essential components: 8mM NaH2PO4, 150mM NaCl, 3mM KCl, 2mM KH2PO4.
- Sterile filtered through 0.2µm filter.
- Ideal for various laboratory applications.
- Convenient formulation saves time and increases productivity.